Fig 1: Validation of the shared DEGs generated from RNA-Seq between the ZC4H2-/- and RNF220-/- NSCs. (A) Venn diagrams of shared DEGs between the ZC4H2-/- and RNF220-/- NSCs. In total, 24 DEGs were shared between the two groups. Z-up/down and R-up/down represent ZC4H2-Seq upregulated/downregulated DEGs and RNF220-Seq upregulated/downregulated DEGs, respectively. (B) Western blot analysis for ZC4H2, RNF220, Cend1, Nrn1, and Six3 protein expression level in WT, ZC4H2-/-, and RNF220-/- NSCs. (C–J) qRT-PCR analysis showing the relative mRNA levels of Cend1, Nrn1, Six3, RNF220, and ZC4H2 in WT, ZC4H2-/-, and RNF220-/-NSCs. ß-actin was used as an internal control. NS represents no significant difference (p > 0.05). ** p < 0.01, *** p < 0.001, two-tailed Student’s t-test. Data represent mean ± SD from three independent biological replicates.